Here we report the development and applicability of multiomics in situ pairwise sequencing MiPseq a method for the simultaneous detection of DNAs RNAs proteins and biomolecules at subcellular resolution

Spatial multiomics at subcellular resolution via highthroughput in

We present deterministic barcoding in tissue for spatial omics sequencing DBiTseq for comapping of mRNAs and proteins in a formaldehydefixed tissue slide via nextgeneration sequencing NGS

Here we report on spatial coindexing of transcriptomes and epitopes for multiomics mapping by highly parallel sequencing spatialCITEseq which uses a cocktail of 200300 ADTs to stain a tissue slide followed by deterministic intissue barcoding of both DNA tags and mRNAs for spatially res

This protocol describes the use of the deterministic barcoding in tissue for spatial omics sequencing platform to construct a multiomics atlas on fixed frozen tissue samples This approach uses a microfluidicbased method to introduce combinatorial DNA oligo barcodes directly to the cells in a tissue section fixed on a glass slide

Keywords Spatial multiomics mouse embryo insitu barcoding high spatial resolution nextgeneration sequencing Graphical Abstract Etoc DBiTseq is a microfluidic based method to deliver barcodes to the surface of a tissue slide to allow for spatial omics sequencing with 10μm pixel size INTRODUCTION

High Spatial Resolution Multi Omics Sequencing Via Pubmed

HighSpatialResolution MultiOmics Sequencing via Deterministic

Together our results provide a singlecell highresolution multiomics view of subnuclear compartments associated genomic loci and their impacts on gene regulation directly within complex tissues The mammalian nucleus is compartmentalized by diverse subnuclear structures

HighSpatialResolution MultiOmics Sequencing via Deterministic

Highresolution spatial multiomics reveals celltype specific PubMed

HighSpatialResolution MultiOmics Sequencing via PubMed

HighSpatialResolution MultiOmics Sequencing via Deterministic

High Spatial Resolution Multi Omics Sequencing Via Pubmed

We present spatialCITEseq for highplex protein and whole transcriptome comapping which was firstly demonstrated for profiling 189 proteins and transcriptome in multiple mouse tissue types

Highplex protein and whole transcriptome comapping at cellular

Spatial multiomics sequencing for fixed tissue via DBiTseq

Spatial multiomics at subcellular resolution via high PubMed

SpatialCITEseq spatially resolved highplex protein and whole

We present deterministic barcoding in tissue for spatial omics sequencing DBiTseq for comapping of mRNAs and proteins in a formaldehydefixed tissue slide via nextgeneration sequencing NGS